On October 10, 1548, Pope Paul III authorized the construction of a General Study Facility. The University College of the Inmaculada Concepción de Nuestra Señora appeared in a document from 1820 confirming that the University College was a pontifical institution.
Don Juan Tellez de Girón, IV Count of Ureña wanted to found a College and University following the model of Alcalá de Henares, where students could obtain degrees that had the same validity as those of Bologna, Salamanca and Alcalá de Henares. The building was financed and endowed exclusively by the Count, with fifteen professorships in the University and eight children in the College, grouped around the faculties of Theology, Law, Medicine and Arts.
In 1824, the establishment was permanently closed. After many years without exercising as such, the University College of Osuna opened its doors to students again in 1993 as part of the University of Seville. It currently offers degrees in Finance and Accounting, Labor Relations and Human Resources, Nursing, Physiotherapy, Primary Education, as well as higher degrees in Finance and Accounting, and Labour Relations and Human Resources.